• FIREPol® 은 매우 진보적이고 열안정성이 있는 DNA 중합효소 입니다.
• 유전자 변형으로 최대 1개월 동안 활성 손실 없이 실온에서 안정성이 향상되었습니다.
• 일상적인 임상 응용 프로그램에 권장됩니다(게놈 DNA에서 최대 3kb 조각)
• 5'→3' polymerase와 5'→3' exonuclease 활성, non-template-dependent terminal transferase 활성을 가지고 있으나
3'→5' exonuclease (proofreading) 활성이 없어 생성된 생성물을 TA- 복제
• FIREPol® 의 정확도는 일반 Taq DNA 중합효소 와 유사합니다 (뉴클레오티드당 오류율 ca 2.5 x10-5)
• 01-01-00500 (500U, 100µl)
• 01-01-01000 (1000U, 200µl)
• 01-01-02000 (2000U, 400µl)
Kit Components
Kit Components |
|
FIREPol® DNA Polymerase |
(5U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. |
FIREPol® 10x Buffer B |
(without Mg2+): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. |
FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. |
|
FIREPol® 10x Buffer BD |
(without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. |
25 mM MgCl₂ |
|
10x Solution S |
an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates). |
• Concentration: 5 U/µl
• Error rate per nucleotide per cycle is app. 2.5 x 10-5
• Accuracy is app. 4 x 104
• Estimated half life at 95ºC is 1.5 hours.
• Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH
• Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
HOT FIREPol® DNA Polymerase
Conventional/End-point PCR ReagentsHOT FIREPol® GC Master Mix
Conventional/End-point PCR ReagentsGeneExplorer 2 x 96-well PCR System
Conventional PCR SystemsSolisFAST® Master Mix, Ready to load
Conventional/End-point PCR ReagentsGeneTouch
Conventional PCR SystemsHOT FIREPol® Blend Master Mix
Conventional/End-point PCR ReagentsLifeTouch
Conventional PCR SystemsFIREPol® Master Mix
Conventional/End-point PCR Reagents