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FIREPol® DNA Polymerase

품명
FIREPol® DNA Polymerase
품번
01-01-00500, 01-01-01000, 01-01-02000
브랜드
Solis Biodyne
Description

• FIREPol® 은 매우 진보적이고 열안정성이 있는 DNA 중합효소 입니다.

• 유전자 변형으로 최대 1개월 동안 활성 손실 없이 실온에서 안정성이 향상되었습니다. 

• 일상적인 임상 응용 프로그램에 권장됩니다(게놈 DNA에서 최대 3kb 조각) 

• 5'→3' polymerase와 5'→3' exonuclease 활성, non-template-dependent terminal transferase 활성을 가지고 있으나 

  3'→5' exonuclease (proofreading) 활성이 없어 생성된 생성물을 TA- 복제 

• FIREPol® 의 정확도는 일반 Taq DNA 중합효소 와 유사합니다 (뉴클레오티드당 오류율 ca 2.5 x10-5)

  

Choose Primers

• 01-01-00500 (500U, 100µl)

• 01-01-01000 (1000U, 200µl)

• 01-01-02000 (2000U, 400µl)  

 

 

Kit Components

Kit Components

FIREPol® DNA Polymerase

(5U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers.

FIREPol® 10x Buffer B

(without Mg2+): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20.

FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.

FIREPol® 10x Buffer BD

(without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4.

25 mM MgCl

 

10x Solution S

an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).

Specification

 

 Concentration: 5 U/µl
 Error rate per nucleotide per cycle is app. 2.5 x 10-5

• Accuracy is app. 4 x 104 

 Estimated half life at 95ºC is 1.5 hours.

 Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 

 Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. 

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